Assuntos
Anfetaminas/farmacologia , Pressão Sanguínea/efeitos dos fármacos , Frequência Cardíaca/efeitos dos fármacos , Fenilpropanolamina/análogos & derivados , p-Hidroxianfetamina/farmacologia , p-Hidroxinorefedrina/farmacologia , Adenilil Ciclases/metabolismo , Agonistas Adrenérgicos beta , Animais , Relação Dose-Resposta a Droga , Masculino , Ratos , Sistema Nervoso Simpático/efeitos dos fármacos , Fatores de Tempo , p-Hidroxianfetamina/sangue , p-Hidroxinorefedrina/sangueRESUMO
Amphetamines, a commonly abused class of drugs, have been detected in various biological specimens, in particular, urine and blood. However, little information is available concerning the detection of these drugs in saliva. This investigation, utilizing the rat salivary secretions, has been attempted to establish the ability of amphetamines to be secreted in saliva and to determine the feasibility of using radioimmunoassay (RIA) for drug detection in saliva. The results of this investigation showed that (1) d-amphetamine and methamphetamine decreased salivary flow, (2) after d-amphetamine RIA tests were demonstrated in both saliva and plasma for a period of fifty minutes, and (3) positive RIA reactions were obtained by the following metamphetamine metabolites: amphetamine, 4-hydroxynorephedrine and 4-hydroxyamphetamine. Methamphetamine and 4-hydroxy-N-methylamphetamine were found to be non-reactive in the radioimmunoassay procedure. The results indicate that saliva could be radioimmunoassayed for the detection of amphetamine or amphetamine derivatives after the administration of either d-amphetamine and methamphetamine.
